Vet Diagnostic - Peste Des Petits Ruminants Antibody Test 

Peste Des Petits Ruminants Virus Antibody Rapid Test Kit

(Whole Blood / Serum / Plasma / Milk)

INTENDED USE

Peste Des Petits Ruminants Virus Antibody Rapid Test Kit  (whole blood / serumr/ plasma/milk) is a solid phase immunochromatographic assay for the rapid,qualitative and dfferentia ldetection of antibody to animal whole blood serum, or plasma. This test provide only a preliminary analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography and mass spectrometry (GC/MS) are the preferred confirmatory methods.

Naked eyes interpretation for Qualitative Results , Quantitative Analysis with machine.

PRINCIPLE

Quantitative or qualitative detection of the titer level of Peste Des Petits Ruminants Virus Antibodies in samples using a double antigen sandwich method. During detection, the Peste Des Petits Ruminants Virus Antibodies in the sample binds to the colloidal gold-coated specific antigen fragment to form an antigen-antibody complex, which flows along the test paper to the other end of the NC membrane. When the complex flows to the T-zone on the membrane, the antigen immobilized on the membrane captures the complex and gradually agglutinates into a red visible T-line, while the unbound colloidal gold antibody flows through the T-zone and is captured by the secondary antibody in the C-zone and forms a visible red C-line.

KIT CONTENTS

STORAGE AND STABILITY

1. Store in a light-proof, dry environment (2-30℃).

2. DO NOT FREEZE or use beyond the expiration date.

3. The shelf life is 18 months.

TEST PROCEDURE

Allow test cassete, specimen, and/ or controls to equilibrate to room temperature (15-30℃) prior to testing.

1. Remove the test cassette from the sealed foil pouch and use it as soon as possible.Best results will be obtained if the assay is performed within one hour.

2. Place the test device on a clean and level surface.

3. Add 100ul (3 drops) sample into the sample well.

4. Wait for the colored line(s) to appear. The result should be read at between 10-15 mintues. It is invalid after 15 minutes.

READ RESULTS WITH NAKED EYES

NEGATIVE:

If only the C band is present, it is negetive.

POSITIVE:

In addition to the presence of C band, T band is developed, it is positive.

INVALID: Reference Line Fails to appear

Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for an invalid result.

Review the procedure and repeat the test with a new test kit. Stop using the test kit immediately if the problem

is not solved and contact your local distributor.

VACCINE ANTIBODY LEVEL ANALYSIS
After vaccination, antibody positivity, uniformity and average titer of antibodies were counted separately in groups according to animal breed, litter size and age.

1. Antibody positivity rate=positive quantity/total quantity*100%.

1). For farms with ideal immunization results, Antibody uniformity(CV%)≤20%. The average antibody titer mostly ranged from 64-256.
2). Sometimes due to immunization procedures, improper timing of immunization or the number of samples is too small, it will also affect the antibody uniformity (>20%).